Water activity, pre-wetting and survival of Pseudomonas sp. ADP immobilized onto zeolite

S. A. Stelting1,2, A. Sunna3, and C. R. Bunt4,5
1Department of Chemical and Process Engineering, University of Canterbury, Private Bag 4800, Christchurch, New Zealand, 2Present address: USDA, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521-2154, 3Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia, 4AgResearch, Private Bag 4749, Lincoln, New Zealand, 4Present address: Faculty of Agriculture and Life Sciences/Department of Agricultural Sciences, Lincoln University, New Zealand

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Summary
Atrazine, a persistent and widely-used herbicide can be degraded by Pseudomonas sp. strain ADP. There is need for a room temperature stable formulation that might help to improve infield delivery and performance. Water activity throughout the study period remained constant (~1.0 aw) for all test samples. Immobilization of Pseudomonas sp. strain ADP onto zeolite using a xanthan gel maintained survival for at least 10 weeks at 25 ÂșC. Approximately 1 log loss by week 7 was observed when xanthan gel was not used. Pre-wetting the zeolite increased the time to 1 log loss to 9 weeks. Formulation, and to some extent moisture does influence the stability of Pseudomonas sp. strain ADP immobilized onto zeolite.

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