S. Jain, S. Kumar, A.K. Agrawal, K. Thanki, U.C. Banerjee
Center for Pharmaceutical Nanotechnology, Dept. of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab, 160062, India
The present study explores the potential of novel Hyaluronic acid-PEI-Cyclodextrin (HA-PEI-CyD) polyplexes, as a non-viral vector for site specific gene delivery. The developed polyplexes exhibited excellent stability against DNase I and serum. Subsequently, HA-PEI-CyD exhibited significantly higher transfection efficiency and cell viability (>90%) in comparison with plain PEI and PEI-CyD polyplexes in HeLa, HEK-293 and MCF-7 cell lines. Furthermore, excellent in-vivo gene expression in tumor bearing animals revealed its suitability as gene delivery reagent.