Enhanced Gene Expression, Transfection and Reduced Cytotoxicity of Novel Hyaluronic Acid-PEICyclodextrin Polyplexes

S. Jain, S. Kumar, A.K. Agrawal, K. Thanki, U.C. Banerjee

Center for Pharmaceutical Nanotechnology, Dept. of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab, 160062, India


The present study explores the potential of novel Hyaluronic acid-PEI-Cyclodextrin (HA-PEI-CyD) polyplexes, as a non-viral vector for site specific gene delivery. The developed polyplexes exhibited excellent stability against DNase I and serum. Subsequently, HA-PEI-CyD exhibited significantly higher transfection efficiency and cell viability (>90%) in comparison with plain PEI and PEI-CyD polyplexes in HeLa, HEK-293 and MCF-7 cell lines. Furthermore, excellent in-vivo gene expression in tumor bearing animals revealed its suitability as gene delivery reagent.

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