Synergistic Effect of M Cell Targeting Peptide and Mucoadhesive Vehicle on Induction of Immune Responses in Oral Vaccine Delivery

B. Singh1,2, S. Maharjan1, 2, T. Jiang1, 2, S.K. Kang1, 2, Y.J. Choi 1, 2, * and C.S. Cho1, 2, *

1Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea;
2Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea

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Summary
Orally ingested pathogens or antigens are taken up by microfold cells (M cells) in the follicle-associated epithelium of Peyer's patches in intestine to initiate protective immunity against infections. Here, we developed a new model of M cell–specific recombinant vaccine which when delivered through oral route using mucoadhesive vehicle induced both mucosal and systemic immunity. Initially, M cell-targeting peptide, selected by phage display technique, was fused to a model lipoprotein antigen (BmpB) by recombinant DNA technology. The recombinant protein (M-BmpB) was loaded into a mucoadhesive vehicle made from thiolated Eudragit (TE) polymer. TE also has ability to protect protein from harsh gastric condition and enzymes in gastrointestinal tract. As a consequence, oral delivery of M-BmpB vaccine through TE vehicle in mouse succeeded to generate both mucosal and systemic immune response against the antigen.

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