Enhanced DNA vaccination using microsphere/polyplex-releasing multi-layered microneedles

Nak Won Kim1, Min Sang Lee1, Jung Eun Lee1, Yong Woo Cho2, Ji Hoon Jeong1,*

1School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
2Department of Chemical Engineering, Hanyang University, Ansan 426-791, Republic of Korea

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Summary
The approach of gene delivery with microneedles has recently been withdrawn much attention. Despite superior capability of microneedles delivering DNA into the dermal tissues, release and cellular uptake of the released DNA after microneedle-based administration have not been clearly studied. In this study, we fabricated polyplex-releasing multilayered microneedles additionally coated with microspheres containing a chemo-attractant for recruiting professional antigen presenting cells such as macrophages. The microspheres can be released from the needle body and localized in the dermis area where chemo-attractant slowly released. The ability of the microneedle-based cutaneous gene delivery system for attracting antigen presenting cells and improving DNA vaccination was tested.

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