Quantitation of sustained release of triptorelin in beagle dog plasma by liquid chromatography-tandem mass spectrometry

Jiangbin Han1, Jiye Sun1, Chunjie Sha1, Jinfeng Zhang1, Yunyun Gai1,Youxin Li1, Wanhui Liu1,2*
1State Key Laboratory of Long-acting and Targeting Drug Delivery System, Shandong Luye Pharmaceutical Co. Ltd, Yantai
264003, P.R. China; 2School of Pharmacy, Yantai University, Yantai 264003, P.R. China

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Summary
A sensitive method based on liquid chromatographytandem mass spectrometry has been developed for the determination of triptorelin in beagle dog plasma using octreotide as the internal standard. Plasma samples were applied to Oasis® HLB solid-phase extraction (SPE) cartridges. Detection with a Qtrap5500 system in the positive ion mode was performed through multiple reaction monitoring of the analyte at m/z 656.5→249.1 and of the I.S. at m/z 510.8→120.1. This method was validated by specificity, linearity, lower limit of quantification (LLOQ), intra and inter-day precision and accuracy, recovery, and stability. Linear calibration curves were obtained in the concentration range of 0.01- 10 ng/mL with the correlation coefficients better than 0.995. Its LLOQ was 0.01 ng/mL. This method has been successfully applied to a pharmacokinetic study for the sustained release of triptorelin in beagle dog plasma after a single intramuscular injection.

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