Cationic Amphiphilic Macromolecules (CAMs)-Lipid Complexes for Intracellular siRNA Delivery

Kathryn E. Uhrich, Li Gu, Leora Nusblat, Charles M. Roth

Rutgers, the State University of New Jersey, 610 Taylor Road, Piscataway, NJ 08854, United States

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Summary
Cationic amphiphilic macromolecules (CAMs) were non-covalently blended with lipids (DOPE and DOTAP) to serve as a siRNA delivery vehicle. By varying weight ratio of CAM to lipid, catonic complexes with different compositions were obtained and their properties were evaluated. Certain CAM-lipid (9N-lipid) with weight ratio of 1:10 was found to have significantly higher transfection efficiency than Lipofectamine. The intracellular trafficking was also applied to elucidate that siRNA escaped endosome at 24 hrs after transfection.

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